Patent · US Active

Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA

US10155929B2 · kind B2 · utility

1Cited by

8References

6Claims

0Family size

Assignee

Allele Biotechnology & Pharmaceuticals, Inc. · US

Inventors

Jiwu Wang · Village of La Jolla, US
Luigi Warren · Boston, US
Yuhui NI · San Diego, US

Key dates

Filing date	May 13, 2013
Grant date	Dec 18, 2018
Priority date	—
Expiry date	May 13, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2510/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.