Rapid antibiotic susceptibility testing system based on bacterial immobilization using gelling agent, antibiotic diffusion and tracking of single bacterial cells
US10155971B2 · kind B2 · utility
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Key dates
| Filing date | Aug 3, 2015 |
| Grant date | Dec 18, 2018 |
| Priority date | — |
| Expiry date | Nov 2, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB29L2031/752
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.