DNA amplification via scissor-like structures (DASL)
US10155977B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Jul 31, 2014 |
| Grant date | Dec 18, 2018 |
| Priority date | — |
| Expiry date | Apr 10, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and systems are provided for amplifying target nucleic acids by means of a polymerase with strand displacement activity, using two or more stem loop primers, each with a 3′-end portion comprising sequence complementary to a target homology site of a target nucleic acid and 5′-end portion with a sequence comprising a stem sequence, a loop sequence and a sequence which is reverse complementary to the stem sequence, the said 5′-end portion being capable of forming a stem loop. Such methods and systems can be used to amplify target nucleic acids isothermally by means of a polymerase with strand displacement activities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.