Prokaryotic 2-component signaling pathways for use as logic gates in mammalian cells
US10160978B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 4, 2015 |
| Grant date | Dec 25, 2018 |
| Priority date | — |
| Expiry date | Jun 4, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to mammalian cells comprising at least one prokaryotic two-component signaling (TCS) pathway comprised of an activator protein A, a response regulator (RR) protein B activated by said protein A, such activation leading to an activated RR protein B, and an output gene C operably linked to a promoter. Transcription from said promoter is activated by activated RR protein B, and the expression of output gene C defines at least a first state (0, no transcription) and a second state (1, detectable transcription). The invention further relates to logic gates designed from such cells, and methods for integrating a plurality of output signals based on the cells and logic gates of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.