Patent · US Active

Method for detecting telomerase via washing-free anchored-extension and telomeric-binding amplification, and kit

US10161005B2 · kind B2 · utility

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6Claims

0Family size

Assignee

ZHEJIANG JFK BIOLOGICAL TECHNOLOGY CO. LTD. · CN

Inventors

Ran Chen · Fremont, US
Xiaozheng Jin · Hangzhou City, CN

Key dates

Filing date	Dec 7, 2015
Grant date	Dec 25, 2018
Priority date	—
Expiry date	Oct 14, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/158
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided is a new method for telomerase amplification—washing-free anchored-extension and telomeric-binding amplification (WATA). The method comprises: utilizing an anchored telomerase primer to conduct telomere TTAGGGG sequence (“G sequence”) extension; hybridizing with a template probe having a universal PCR primer sequence and six units of telomere CCCTAA sequence (“C sequence”) on the extended G sequence; removing the uncombined template probe via an enzyme digestion reaction; and the template probe combined with the G sequence conducts PCR reaction, the amplification product being a peculiar DNA fragment of a fixed length.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.