Methods for detection of anti-cytomegalovirus neutralizing antibodies
US10161010B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 2018 |
| Grant date | Dec 25, 2018 |
| Priority date | — |
| Expiry date | May 29, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2469/20
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present disclosure provides methods useful for determining levels of HCMV infection in host cells and, by extension, determining levels of neutralizing antibodies present in a sample. The present disclosure encompasses the recognition that HCMV viruses that have a fluorescent moiety permit detection of viral infection (e.g., by assessing fluorescence in cells after contacting the host cell with the virus). In some embodiments, levels of HCMV infection are determined by fluorescence detection where the virus has been preincubated with a test sample (e.g., a serum sample) from a subject. In some embodiments, the subject has been administered a candidate HCMV vaccine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.