Fluorescent microscopic imaging method and apparatus
US10161873B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 20, 2016 |
| Grant date | Dec 25, 2018 |
| Priority date | — |
| Expiry date | May 20, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/36
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence microscopic imaging method includes: after a to-be-detected sample plate is placed, lightening, according to experimental requirements, at least one monochromatic fluorescence excitation light source with a same color among multiple monochromatic fluorescence excitation light sources as a target light source, where monochromatic fluorescence excitation light emitted by each monochromatic fluorescence excitation light source obliquely enters a preset detection region of the to-be-detected sample plate; collecting, at a side of the to-be-detected sample plate facing away from the target light source, fluorescence of particles within the preset detection region excited by irradiation of monochromatic fluorescence excitation light emitted by the target light source, and magnifying the preset detection region a preset number of times; filtering the excited fluorescence of the particles within the preset detection region; and acquiring a fluorescence image of the preset detection region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.