Patent · US Active

Microfluidic systems and methods for hydrodynamic microvortical cell rotation in live-cell computed tomography

US10162162B2 · kind B2 · utility

9Cited by

5References

17Claims

0Family size

Assignee

ARIZONA BOARD OF REGENTS, A BODY CORPORATE OF THE STATE OF ARIZONA ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY · US

Inventors

Hong Wang · Cypress, US
Deirdre Meldrum · Phoenix, US
Rishabh Shetty · Tempe, US
Laimonas Kelbauskas · Gilbert, US
Shih-Hui (Joseph) Chao · Phoenix, US
Roger Johnson · Phoenix, US
Jakrey Myers · Scottsdale, US
Samantha Chan · Toronto, CA

Key dates

Filing date	Sep 22, 2015
Grant date	Dec 25, 2018
Priority date	—
Expiry date	Oct 9, 2035

Classification

Technology area (CPC B)Performing Operations; Transporting
CPC primaryB01L2400/0487
WIPO fieldOptics
WIPO sectorInstruments

Abstract

Microfluidic devices for 3D hydrodynamic microvortical rotation of at least one live single cell or cell cluster, systems incorporating the devices, and methods of fabricating and using the devices and systems, are provided. A microfluidic chip rotates at least one live single cell or cell cluster in a microvortex about a stable rotation axis perpendicular to an optical axis within a chamber having a trapezoidal cross-sectional shape located below a flow channel. An optical trap may be used to position the cell or cells with the microvortex, and the cell or cells may be subject to live-cell or cell cluster computer tomography imaging.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.