Luminophore-labeled molecules coupled with particles for microarray-based assays

US10167499B2 · kind B2 · utility

Assignees

• CAPITALBIO TECHNOLOGY CORPORATION · CN
• TSINGHUA UNIVERSITY · CN

Inventors

• Yang Li · Shanghai, CN
• Guanbin Zhang · Beijing, CN
• Di Jiang · Beijing, CN
• Guangxin Xiang · Beijing, CN
• Wanli Xing · Beijing, CN
• Jing Cheng · Beijing, CN

Key dates

• Filing date: Dec 2, 2014
• Grant date: Jan 1, 2019
• Expiry date: Dec 2, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules.