Rapid and sensitive method for detection of biological targets
US10168335B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 26, 2016 |
| Grant date | Jan 1, 2019 |
| Priority date | — |
| Expiry date | May 26, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/908
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.