Methods of functionalization and reagents used in such methods using an aza-isatoic anhydride or a derivative thereof, biological molecules thus treated and kits
US10174068B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 26, 2013 |
| Grant date | Jan 8, 2019 |
| Priority date | — |
| Expiry date | Mar 25, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6825
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to a method of functionalization of at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which includes the following steps: a) providing at least: one binding molecule consisting of an aza-isatoic anhydride or a derivative thereof, one group of interest, and one linkage joining the binding molecule to the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group carried: in position 2′ of the ribose of one of the RNA nucleotides, and/or in position(s) 2′ and/or 3′ of the ribose of the nucleotide at the terminal 3′ end of the RNA, and obtaining an aza-anthranilate that joins, by means of the linkage, the RNA to the group of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.