Patent · US Active

Method of estimating the amount of a methylated locus in a sample

US10174383B2 · kind B2 · utility

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1References

17Claims

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Assignee

VANADIS DIAGNOSTICS · SE

Inventors

Carl Oscar Fredrik Dahl · Sigtuna, SE
Olof John Ericsson · Uppsala, SE
Johan Baner · San Francisco, US

Key dates

Filing date	Jul 30, 2015
Grant date	Jan 8, 2019
Priority date	—
Expiry date	Feb 10, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/158
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.

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