Cell-targeting nanoparticles comprising polynucleotide agents and uses thereof
US10179113B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 10, 2017 |
| Grant date | Jan 15, 2019 |
| Priority date | — |
| Expiry date | Jan 10, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2991
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of generating a particle is disclosed, the particle being for delivery of a polynucleotide to a target cell. The method comprises (a) contacting the polynucleotide with a composition comprising cationic molecules, wherein the cationic molecules condense the polynucleotide by electrostatic interactions to generate a complex, wherein the cationic molecules are not comprised in a liposome; and (b) covalently binding the complex to a targeting moiety at a pH equal to or below about 4.5, thereby generating the particle for delivery of the polynucleotide agent to the target cell. Use of the particles and compositions comprising same are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.