Conditioned media and methods of making a conditioned media
US10179900B2 · kind B2 · utility
1Cited by
101References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 19, 2008 |
| Grant date | Jan 15, 2019 |
| Priority date | — |
| Expiry date | Jun 17, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/95
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A stable and scalable process is provided to manufacture reduced serum umbilical cord tissue-derived (UTC)-conditioned media. The method includes the culture of a UTC under reduced serum conditions. Subsequently, the UTC is washed and grown in serum-free basal media. After approximately 24 hours, the conditioned media is collected, filtered and concentrated by use of an approximately 5 kDa or similar cut-off membrane.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.