Liposomal particles, methods of making same and uses thereof

US10182988B2 · kind B2 · utility

Assignees

• Northwestern University · US
• EXICURE, INC. · US

Inventors

• Chad A. Mirkin · Wilmette, US
• SonBinh T. Nguyen · Evanston, US
• Resham Singh Banga · Chicago, US
• Natalia Chernyak · Evanston, US
• Sergei Gryaznov · San Mateo, US
• Aleksandar Filip Radovic-Moreno · Boston, US
• Christopher C. Mader · Cambridge, US

Key dates

• Filing date: Dec 3, 2014
• Grant date: Jan 22, 2019
• Expiry date: Dec 3, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2320/32
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Liposomes termed as small unilamellar vesicles (SUVs), can be synthesized in the 20-50 nm size range, but encounter challenges such as instability and aggregation leading to inter-particle fusion. This limits their use as a therapeutic delivery agent. Increasing the surface negative charge of SUVs, via the attachment of anionic entities such as DNA/RNA, increases the colloidal stability of these vesicles. Additionally, the dense spherical arrangement and radial orientation of nucleic acids exhibits unique chemical and biological properties, unlike their linear counterparts. These liposomal particles, are non-toxic and though anionic, can efficiently enter cells without the aid of ancillary cationic transfection agents in a non-immunogenic fashion. These exceptional properties allow their use as delivery agents for gene regulation in different therapies and offer an alternative platform to metal core spherical nucleic acids.