Light-switchable gene expression system and the methods for controlling gene expression in prokaryotic bacterium

US10184126B2 · kind B2 · utility

Assignee

• East China University of Science and Technology · CN

Inventors

• Yi Yang · Shanghai, CN
• Xianjun Chen · Shanghai, CN
• Zhengcai Ma · Shanghai, CN
• Renmei Liu · Shanghai, CN

Key dates

• Filing date: Aug 1, 2013
• Grant date: Jan 22, 2019
• Expiry date: Aug 1, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regu…