Genetic transformation of bifidobacteria
US10184127B2 · kind B2 · utility
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9Claims
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Key dates
| Filing date | Dec 2, 2013 |
| Grant date | Jan 22, 2019 |
| Priority date | — |
| Expiry date | Dec 6, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y201/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention concerns a method for genetically transforming a Bifidobacterium strain comprising a step of methylation of a shuttle vector in an E. coli or a Gram-positive bacterium strain by two type II DNA methyltransferases from a Bifidobacterium: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence RTCAGG and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence GGWCC. The present invention also concerns genetic tools and culture media useful for carrying out said method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.