Materials and methods for production of bi-functional fatty acids in recombinant bacteria
US10184140B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 30, 2015 |
| Grant date | Jan 22, 2019 |
| Priority date | — |
| Expiry date | Dec 20, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y203/0118
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more ω- or ω-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the ω- or ω-1 functionalized acyl-CoAs as a substrate; a method of producing a ω-1 hydroxy branched fatty acid, a ω-1 branched fatty acid, or a combination thereof by culturing a mutant E. coli, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant E. coli, a method of making the mutant, a culture comprising the mutant, and a composition comprising ω-1 hydroxy branched fatty acids, a ω-1 branched fatty acids, or a combination thereof obtained from the culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.