Patent · US Active

Affinity chromatography matrix

US10189891B2 · kind B2 · utility

0Cited by

9References

40Claims

0Family size

Assignee

GE Healthcare BioProcess R&D AB · SE

Inventors

Mats Ander · Uppsala, SE
Goran Bauren · Uppsala, SE
Tomas Bjorkman · Uppsala, SE
Per-Mikael Aberg · Uppsala, SE
Gustav Rodrigo · Uppsala, SE

Key dates

Filing date	Mar 26, 2013
Grant date	Jan 29, 2019
Priority date	—
Expiry date	Jan 22, 2035

Classification

Technology area (CPC B)Performing Operations; Transporting
CPC primaryB01J2220/80
WIPO fieldChemical engineering
WIPO sectorChemistry

Abstract

The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.