Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
US10190153B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 7, 2014 |
| Grant date | Jan 29, 2019 |
| Priority date | — |
| Expiry date | Jul 19, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/707
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a rapid and highly effective method for the preparation of biological samples for detection of both DNA and RNA target molecules. The method comprises treatment of the sample with a clay mineral followed by lysis of the sample with an alkaline solution. The method is particularly suited for preparing complex biological samples, such as blood or plasma, for the simultaneous detection of DNA and RNA targets. Samples prepared by the method of the invention may be directly used as targets in PCR amplification. The method of the invention may conveniently be coupled with further steps and devices to perform molecular analyses for diagnostic and other applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.