Method for detecting and identifying enterohemorrhagic Escherichia coli

US10190175B2 · kind B2 · utility

Assignees

• Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et due Travail · FR
• Bundesinstitut Fur Risikobewertung · DE

Inventors

• Patrick Fach · Créteil, FR
• Sabine Delannoy · Cachan, FR
• Lothar Beutin · Berlin, DE

Key dates

• Filing date: Jun 14, 2013
• Grant date: Jan 29, 2019
• Expiry date: Aug 26, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/158
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to methods for predicting whether a sample contains enterohemorrhagic Escherichia coli (EHEC) of at least one of EHEC O157:[H7], O145:[H28], O103:[H2], O111:[H8], O121:[H19], O26: [H11], O45:[H2] or O104: [H4] serotypes, and for identifying said serotypes, through detection of gene espK in association with at least one of the genetic markers Z1151, Z1153, Z1154, Z1155, Z1156, Z6065, Z2098, ureD or espV and/or through detection of serotype-specific CRISPR sequences.