Methods of and systems for improved detection sensitivity of assays
US10191037B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 8, 2013 |
| Grant date | Jan 29, 2019 |
| Priority date | — |
| Expiry date | Mar 21, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0851
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems and methods are disclosed for improved detection sensitivity of assays. The systems include an upper plate that is inserted into a well of testing well, such as a base microtiter plate. For bottom detection, a coating, including polymer coatings or membrane coatings, is applied to an insert portion of the upper plate, and the base plate includes transparent windows. In bottom detection, a detector will image from below the base plate. Alternatively, for top detection, the coating is applied to the well of the base plate, and the upper plate includes transparent windows. In top detection, a detector will image from above the upper plate. Analysis features, including capture antibody features or antigen features, can be printed on the coating surface for forward-phase assays or reverse-phase assays, respectively. Methods for making and using the same are disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.