Method for forming hydrogel arrays using surfaces with differential wettability

US10195313B2 · kind B2 · utility

Assignee

• Wisconsin Alumni Research Foundation · US

Inventors

• William L. Murphy · Waunakee, US
• Ngoc Nhi Le · Norcross, US
• Stefan Zorn · Deggendorf, DE
• Michael P. Schwartz · Madison, US
• Eric Nguyen · Madison, US

Key dates

• Filing date: Jul 24, 2014
• Grant date: Feb 5, 2019
• Expiry date: Oct 21, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2610/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Patterned hydrogel arrays and methods of preparing patterned hydrogel arrays are disclosed. Advantageously, the methods used to prepare the patterned hydrogel arrays allow for controlling individual hydrogel spot conditions such as hydrogel spot modulus, hydrogel spot ligand identity and hydrogel spot ligand density, which allows for preparing a wide range of hydrogel spots in a single array format. Patterned hydrogel arrays can also be formed to include hydrogel-free pools surrounded by hydrogel. Additionally, the patterned hydrogel arrays of the present disclosure support the culture of a range of cell types. The patterned hydrogel arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.