Method and kit for detecting 1,25-dihydroxyvitamin D and related antibodies
US10196449B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 27, 2014 |
| Grant date | Feb 5, 2019 |
| Priority date | — |
| Expiry date | Oct 3, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/32
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method of the invention, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method of the invention. The assay of the invention is preferably a sandwich immunoassay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.