Patent · US Active

Protein production

US10196666B2 · kind B2 · utility

0Cited by

3References

19Claims

0Family size

Assignee

Thermo Fisher Scientific Baltics UAB · LT

Inventors

Juozas {hacek over (S)}iurkus · Kaišiadorys, LT
Peter Neubauer · Oulu, FI

Key dates

Filing date	Jul 1, 2016
Grant date	Feb 5, 2019
Priority date	—
Expiry date	Aug 20, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N1/20
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.