Reagents and methods for screening MPS I, II, IIIA, IIIB, IVA, VI, and VII
US10196668B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 5, 2014 |
| Grant date | Feb 5, 2019 |
| Priority date | — |
| Expiry date | Aug 18, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IV A, MPS-VI, and MPS VII. In one aspect, the invention provides methods for assaying one or more enzymes associated with a lysosomal storage disease. In a first embodiment, the method includes: (a) contacting a sample with a first solution to provide a solution comprising one or more lysosomal enzymes; (b) contacting the one or more lysosomal enzymes in solution with an enzyme substrate for each lysosomal enzyme to be analyzed and incubating the substrates with the enzymes for a time sufficient to provide a solution comprising an enzyme product for each lysosomal enzyme present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.