Multiphase nucleic acid amplification

US10196674B2 · kind B2 · utility

Assignee

• GEN-PROBE INCORPORATED · US

Inventors

• Norman C. Nelson · San Diego, US
• Lyle J. Arnold, Jr. · Poway, US
• Lizhong DAI · San Diego, US
• Steven Phelps · San Diego, US
• Jijumon Chelliserry · San Diego, US

Key dates

• Filing date: Sep 16, 2015
• Grant date: Feb 5, 2019
• Expiry date: Nov 4, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.