Microfluidic system and method for isolating and quantifying at least one sub-population of cells from a population of cells
US10197570B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 25, 2014 |
| Grant date | Feb 5, 2019 |
| Priority date | — |
| Expiry date | Sep 4, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/70596
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are a microfluidic system (1) configured to receive cell populations and further configured to simultaneously isolate and quantify at least one sub-population of cells for each cell population, and related methods of using the system. The system comprises a substrate having networks of microchannels comprising a first sorting unit configured to isolate, by magnetic attraction, cells of interest in the population in at least one first sorting microchannel. The network comprises a second unit for simultaneous sorting and counting comprising at least one second sorting microchannel defined by a closed wall having an inner face provided with at least one functionalised electrode configured to trap a sub-population. The second unit further comprising means for counting the sub-population by impedance spectroscopy. The second sorting microchannel can have at least one pair of opposing functionalised electrodes and at least one pair of second micro-coils for trapping the cells of interest arranged in the wall facing the electrodes and controlling successive attraction/release cycles that alternate between the micro-coils.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.