System and method for image acquisition using supervised high quality imaging
US10200625B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 2015 |
| Grant date | Feb 5, 2019 |
| Priority date | — |
| Expiry date | Jul 6, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated. Upon a determination that a predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, or when the predetermined upper threshold for the time interval for image acquisition is elapsed the system provides a final image for the given time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.