Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
US10202630B2 · kind B2 · utility
2Cited by
2References
27Claims
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Key dates
| Filing date | Mar 17, 2014 |
| Grant date | Feb 12, 2019 |
| Priority date | — |
| Expiry date | Nov 20, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2523/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.