Single cell analysis of T cells using high-throughput multiplex amplification and deep sequencing

US10202640B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Mark M. Davis · Mountain View, US
• Jacob Glanville · San Francisco, US
• Arnold Han · Los Altos Hills, US

Key dates

• Filing date: Apr 30, 2015
• Grant date: Feb 12, 2019
• Expiry date: Sep 7, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and oligonucleotide reagents for analyzing individual T cells are disclosed. In particular, the present disclosure provides methods for analyzing individual T cells using high-throughput multiplex amplification and deep sequencing of nucleic acids encoding T cell receptors (TCRs) and various other T cell phenotypic markers. The present disclosure further provides methods of reconstituting TCRs from individual T cells for functional studies, ligand discovery, or screening therapeutics.