Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US10207240B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 3, 2010 |
| Grant date | Feb 19, 2019 |
| Priority date | — |
| Expiry date | Nov 3, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/629
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.