Method for cultivating cells in adhesion culture by using a cell culture carrier in capsule form, and cell culture carrier therefor

US10208281B2 · kind B2 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Daniel Rapoport · Stade, DE
• Miriam Voigt · Bad Schwartau, DE
• Charlotte WALTER · Stade, DE

Key dates

• Filing date: Sep 22, 2014
• Grant date: Feb 19, 2019
• Expiry date: Sep 22, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2537/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for cultivating cells in adhesion culture, comprising at least the following steps: a) dissolving or suspending a cross-linkable, biocompatible material having adhesion points for cells in a cell culture medium; b) suspending cells in the cell culture medium, which contains the cross-linkable, biocompatible material, or in a medium that contains at least one component that is required for the cross-linking of the cross-linkable, biocompatible material; c) introducing the cell suspension into a medium in drops under conditions that initiate or permit the cross-linking of the biocompatible material, wherein either the cell suspension or the medium into which the cell suspension is introduced in drops contains the cross-linkable biocompatible material; d) forming stable, preferably porous capsules from cross-linked biocompatible material, which capsules contain incorporated adherent cells; e) proliferating the adherent cells in the capsules for a specified time period; f) breaking up the capsule material by means of a physical or chemical stimulus and releasing the cells as a cell suspension. In an especially preferred embodiment of the invention, the…