Patent · US Active

Process for the purification of a growth factor protein

US10214575B2 · kind B2 · utility

0Cited by

4References

17Claims

0Family size

Assignee

OCTAPHARMA AG · CH

Inventors

Gustav Gilljam · Älta, SE
Stefan Winge · Kramfors, SE
Maya Tiemeyer · Ladenburg, DE

Key dates

Filing date	Sep 2, 2016
Grant date	Feb 26, 2019
Priority date	—
Expiry date	Sep 2, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/575
WIPO fieldChemical engineering
WIPO sectorChemistry

Abstract

A method of purifying a Growth Factor Protein in a purification sequence employing chromatography is provided. The method comprises performing at least one chromatography step using a multimodal resin, binding the Growth Factor Protein to the multimodal resin at a pH from 4 to 6.2, and eluting the Growth Factor Protein at a pH in the range of from 5.5 to 6.5. The elution of Growth Factor Protein is improved by addition of arginine and/or NaCl in the eluting buffer. Optionally the multimodal resin step is followed by a yeast derived affinity ligand resin step, which results in a purity of the product greater than 90%.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.