Patent · US Active

Mesenchymal precursor cell

US10220057B2 · kind B2 · utility

0Cited by

0References

10Claims

0Family size

Assignee

MESOBLAST, INC. · US

Inventors

Paul Simmons · Melbourne, AU
Stan Gronthos · Adelaide, AU
Andrew Zannetino · Pimba, AU

Key dates

Filing date	Sep 10, 2013
Grant date	Mar 5, 2019
Priority date	—
Expiry date	Sep 10, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2501/39
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.