Solubility optimization of immunobinders
US10221237B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Dec 14, 2016 |
| Grant date | Mar 5, 2019 |
| Priority date | — |
| Expiry date | Dec 14, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/94
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides methods of using sequence based analysis and rational strategies to improve the solubility of immunobinders, and in particular of single chain antibodies (scFvs). The invention provides methods of engineering immunobinders, and in particular scFvs, by performing one or more substitutions with hydrophilic residues identified by analysis of a database of selected, stable scFv sequences. The invention also provides immunobinders with optimized solubility prepared according to the engineering methods of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.