Isotachophoresis enhanced isothermal nucleic acid amplification
US10227634B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 14, 2016 |
| Grant date | Mar 12, 2019 |
| Priority date | — |
| Expiry date | Apr 14, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44795
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates generally to the integration of isotachophoresis (ITP) and isothermal nucleic acid amplification methods such as recombinase polymerase amplification (RPA). One aspect of the disclosure relates to a method for concentrating and amplifying a nucleic acid, the method including an isotachophoresis device, the isotachophoresis device including a porous matrix, and first and second electrodes, having a leading electrolyte, a trailing electrolyte and a set of isothermal nucleic acid amplification reaction reagents disposed in the porous matrix as described herein, and applying a voltage across the first electrode and the second electrode for a time sufficient to provide a first isotachophoresis (ITP) plug comprising an amplification product of the nucleic acid, wherein the concentration of the nucleic acid is substantially higher in the first FTP plug than in the first and/or second fluids outside of the first ITP plug.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.