Nucleotide polymorphism detection method
US10227638B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 2, 2015 |
| Grant date | Mar 12, 2019 |
| Priority date | — |
| Expiry date | Oct 11, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2561/125
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for characterising a DNA analyte comprised of one or more polynucleotide types characteristic of a site of nucleotide polymorphism each of which includes a target region having the formula -X-Y-Z- wherein X and Z are respectively first and second characteristic flanking oligonucleotide regions and Y is one of the variants constituting the site is provided. The method is characterised by the steps of; (a) reacting a single-stranded oligonucleotide including the target region derived from at least one of the polynucleotide types with a set of unused probes comprised of (i) a single-stranded first aptamer terminating at its 3′ end in a sequence complementary to that of -X or -X-Y and (ii) one or more second single-stranded aptamers terminating at their 5′ end in a sequence complementary to that of -Z-Y or -Z (as the case may be) and labelled with detectable elements which are in an undetectable state in the presence of a ligase to create a substantially double-stranded used probe comprised of the oligonucleotide, first aptamer and one of the second aptamers; (b) wholly or in part digesting the used probe with an exonuclease or polymerase exhibiting exonuclease activity in a 3…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.