RNase H-based assays utilizing modified RNA monomers
US10227641B2 · kind B2 · utility
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55Claims
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Key dates
| Filing date | Dec 2, 2013 |
| Grant date | Mar 12, 2019 |
| Priority date | — |
| Expiry date | Nov 9, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/26004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mg++ cation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.