DNA sequencing using controlled strand displacement

US10227647B2 · kind B2 · utility

Assignee

• Complete Genomics, Inc. · US

Inventors

• Rongqin Ke · Mountain View, US
• Snezana Drmanac · Nanhu, CN
• Radoje Drmanac · Los Altos Hills, US
• Guangyang Cai · Milpitas, US
• Matthew J. Callow · Redwood City, US

Key dates

• Filing date: Feb 10, 2016
• Grant date: Mar 12, 2019
• Expiry date: Jul 31, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.