Systems and method for high-throughput testing

US10232336B2 · kind B2 · utility

Assignees

• The Charles Stark Draper Laboratory, Inc. · US
• The Brigham and Women's Hospital, Inc. · US

Inventors

• Guillermo Garcia-Cardena · Boston, US
• Peter Mack · Chapel Hill, US
• Jeffrey T. Borenstein · Holliston, US
• Ahmad S. Khalil · Lexington, US
• Eli Weinberg · Needham, US
• Jason O. Fiering · Boston, US
• Ernest S. Kim · Cambridge, US
• William James Louis Adams · DeLand, US
• Mitchell L. Hansberry · Framingham, US
• Stephen L. Bellio · Newton, US

Key dates

• Filing date: Nov 6, 2014
• Grant date: Mar 19, 2019
• Expiry date: Jun 8, 2037

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2219/00587
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The systems and methods described herein relate to a high-throughput flow apparatus. The apparatus is used with an array of wells, and is configured to impart a predetermined shear stress on cells cultured within each of the wells of the array of wells. The apparatus includes a plurality of mechanical tips. The plurality of mechanical tips each includes a head with a hemispheroid shape. The apparatus also includes a motor associated with at least one of plurality of mechanical tips. The motor is configured to drive the plurality of mechanical tips to impart the shear stress pattern in each of the wells.