Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
US10233440B2 · kind B2 · utility
2Cited by
2References
31Claims
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Key dates
| Filing date | Sep 26, 2013 |
| Grant date | Mar 19, 2019 |
| Priority date | — |
| Expiry date | Mar 26, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/06
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.