Dot detection, color classification of dots and counting of color classified dots
US10235559B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 25, 2017 |
| Grant date | Mar 19, 2019 |
| Priority date | — |
| Expiry date | Jul 26, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30096
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
The present disclosures relates to a method of detecting, classifying, and counting dots in an image of a tissue specimen comprising detecting dots in an image of the tissue sample that meet criteria for absorbance strength, black unmixed image channel strength, red unmixed image channel strength, and a difference of Gaussian threshold, wherein the detected dots correspond to in situ hybridization signals in the tissue samples; classifying the detected dots as belonging to a black in situ hybridization signal or to a red in situ hybridization signal; and calculating a ratio of those dots belonging to the black in situ hybridization signal and those belonging to the red in situ hybridization signal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.