Method of preparation of nanopore and uses thereof

US10246479B2 · kind B2 · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• Government of the United States of America, as represented by the Secretary of Commerce · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Shiv Kumar · Montgomery, US
• Chuanjuan Tao · Fort Lee, US
• Minchen Chien · Tenafly, US
• James J. Russo · New York, US
• John J. Kasianowicz · Gaithersburg, US
• Joseph W. Robertson · Washington, US

Key dates

• Filing date: Apr 8, 2013
• Grant date: Apr 2, 2019
• Expiry date: May 30, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/9125
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.