Purification of recombinantly produced polypeptides
US10259842B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | May 13, 2014 |
| Grant date | Apr 16, 2019 |
| Priority date | — |
| Expiry date | Apr 26, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC11D7/265
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein is a method for separating a recombinantly produced polypeptide from host cell protein. The method includes a step of loading a clarified cell culture supernatant that includes the recombinantly produced polypeptide and the HCP onto a Protein A chromatography column and washing the Protein A chromatography column with a wash buffer comprising a fatty acid having a chain length of at least about 6 carbon atoms, or a fatty acid salt thereof to remove HCP and then recovering the recombinantly produced polypeptide.
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