Delivery of cargo proteins via ARRDC1-mediated microvesicles (ARMMs)

US10260055B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Quan Lu · Shanghai, CN
• Qiyu Wang · Boston, US
• Stanley N. Cohen · Stanford, US

Key dates

• Filing date: Nov 10, 2017
• Grant date: Apr 16, 2019
• Expiry date: Nov 10, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2810/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods, systems, compositions and strategies for the delivery of WW domain-containing fusion proteins into cells in vivo, ex vivo, or in vitro via ARMMs are provided. Methods, systems, compositions and strategies for the delivery of cargo proteins, such as transcription factors, tumor suppressors, developmental regulators, growth factors, metastasis suppressors, pro-apoptotic proteins, nucleases, recombinases, and reprogramming factors into cells in vivo, ex vivo, or in vitro via fusion to ARMM associated proteins (e.g., ARRDC1 or TSG101) are also provided.