Cleavage of fucose in N-glycans
US10260056B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Feb 12, 2018 |
| Grant date | Apr 16, 2019 |
| Priority date | — |
| Expiry date | Feb 12, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is an α-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The α-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the α-fucosidase are provided, as well as a method for analyzing glycoproteins. The α-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.