Accelerated isothermal amplification of DNA
US10260090B2 · kind B2 · utility
3Cited by
2References
20Claims
0Family size
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Key dates
| Filing date | Jul 18, 2016 |
| Grant date | Apr 16, 2019 |
| Priority date | — |
| Expiry date | Jul 21, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.