DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators

US10260094B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Dae Hyun Kim · Anmyeon-eup, KR
• Jia Guo · Chandler, US
• Qinglin Meng · Sunnyvale, US
• Zengmin Li · Great Neck, US
• Huanyan Cao · Jiangyin, CN

Key dates

• Filing date: Aug 6, 2015
• Grant date: Apr 16, 2019
• Expiry date: Jan 17, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/107
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogs or 3′ modified deoxyinosine triphosphate analogs, and 3′ modified dideoxynucleotide analogs having a detectable marker attached to a base thereof.