Glutathione-detecting fluorescent probe

US10261090B2 · kind B2 · utility

Assignee

• THE UNIVERSITY OF TOKYO · JP

Inventors

• Yasuteru Urano · Tokyo, JP
• Mako Kamiya · Tokyo, JP
• Keitaro Umezawa · Tokyo, JP
• Masafumi Yoshida · Tokyo, JP

Key dates

• Filing date: Feb 24, 2015
• Grant date: Apr 16, 2019
• Expiry date: May 8, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6441
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

[Problem] To provide the following: a novel fluorescent probe for detecting a compound, such as glutathione, that contains a —SH group; a detection method using said fluorescent probe; and a detection kit containing said probe.[Solution] A fluorescent probe for detecting a compound containing an —SH group, wherein the fluorescent probe comprises a compound represented by formula (I) or a salt thereof (In the formula, X represents Si(Ra)(Rb), Ge(Ra)(Rb), Sn(Ra)(Rb), C(Ra)(Rb), or O (wherein Ra and Rb each independently represent a hydrogen atom or alkyl group); R1 represents a hydrogen atom, or 1-4 identical or different substituents independently selected from the group consisting of a cyano group, alkyl group, carboxyl group, ester group, alkoxy group, amide group, and azide group, each of which may be optionally substituted; R2 represents a hydrogen atom, halogen atom, hydroxyl group, cyano group, or an alkyl group, alkynyl group, alkoxy group, aryl, or heteroaryl, each of which may be optionally substituted; R3 and R4 each independently represent a hydrogen atom or 1-3 identical or different substituents independently selected from the group consisting of a hydroxyl group, halog…